T cell Purification

Dina Fonseca dfonseca at mail1.sas.upenn.edu
Sat Oct 1 16:10:43 EST 1994

: The only T cell that would be unresponsive to PHA would be an anergized
: one. Is this what you're asking for?

: Do you want helper CD4s or cytotoxic CD8s or both?
: You could select for these markers. As far as negative selection,
: you're in for a long experiment.  There's over 200 other non-T cells
: you'll have to negatively select out for removal.
:  How about + selection for CD40-ligand?
: --                          ^^^^^^^^^^^
:                                              psycler at netcom.com
I assume these are human T cells.
I did'nt know resting human T cells expressed CD40L.
Is this true?
A Bhandoola

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