T cell Purification

Luis Diaz cascas at umich.edu
Sat Oct 1 10:48:29 EST 1994


I am interested in a fast and efficient protocol for obtaining a very
pure T lymphocytes from peripheral blood.  Negative selection would be
prefered - as to avoid activation - and the cells must not be responsive
to superantigens or PHA stimualtion.  If anyone has any good ideas, other
than standard E+ rossetting or  positive selection, please let me know.

Luis Diaz
Medical Student
Univ. of Michigan



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