T cell Purification

[Luis Diaz]

I am interested in a fast and efficient protocol for obtaining a very
pure T lymphocytes from peripheral blood.  Negative selection would be
prefered - as to avoid activation - and the cells must not be responsive
to superantigens or PHA stimualtion.  If anyone has any good ideas, other
than standard E+ rossetting or  positive selection, please let me know.

Luis Diaz
Medical Student
Univ. of Michigan