Antibodies to SDS-PAGE purified proteins

J. Aphale japhale at gpu.utcc.utoronto.ca
Mon Oct 3 14:54:47 EST 1994


Hello Netters:

	Could someone provide me information on obtaining antibodies
to SDS-PAGE purified (separated?) proteins.

	I am running a non-denatyring SDS-PAGE to separate a 30 kD
protein from contaminants and wish to raise antibodies against this
protein in rabbits.  The protein is not glycosylated.

	I need to know hoew much protein is needed per band/s in order
to get a good antibody response and also the method of immunization.
Could you please reply to my e-mail address japhale at utcc.utoronto.ca ?

	Thanks in advance.


						Jayant




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