Antibody titering for flow cytometry

John Ladasky ladasky at leland.Stanford.EDU
Wed Oct 5 13:07:07 EST 1994


Greetings, everyone,

	Using a flow cytometer and an antibody at various dilutions, I've
just analyzed the fluorescence intensity of a particular cell-antibody 
combination.  I now have a nice, asymptotic curve.

			    Mean
	ug MAb added	Fluorescence
	----------------------------
	    0.00	       8
	    0.63	     364
	    1.25	     541
	    2.50	     812
	    5.00	    1050
	
	Now what?  At what level is an antibody considered to be "titered"?
Any advice or references would be greatly appreciated.  Thanks!


-- 
Unique ID : Ladasky, John Joseph Jr.
Title     : BA Biochemistry, U.C. Berkeley, 1989
Location  : Stanford University Dept. of Cell Biology, Fairchild D-105
Keywords  : immunology, music, running, Green



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