Antibody titering for flow cytometry
Richard R. Hardy
hardy at mighty.fccc.edu
Thu Oct 6 13:00:17 EST 1994
In article <36uq0b$58m at nntp.Stanford.EDU>, ladasky at leland.Stanford.EDU
(John Ladasky) wrote:
> Greetings, everyone,
> Using a flow cytometer and an antibody at various dilutions, I've
> just analyzed the fluorescence intensity of a particular cell-antibody
> combination. I now have a nice, asymptotic curve.
> ug MAb added Fluorescence
> 0.00 8
> 0.63 364
> 1.25 541
> 2.50 812
> 5.00 1050
> Now what? At what level is an antibody considered to be "titered"?
> Any advice or references would be greatly appreciated. Thanks!
You're close to some REAL experts on Flow Cytometry and cell surface
immunofluorescence - why not visit someone in the Herzenberg lab / FACS
development group (in the basement of CMGM) and show them your plots?
Aaron Kantor may still be around... We could give advice and recommend
chapters/books, but it's still good to see the raw data - i.e., to
recognize potential pitfalls like problem backgrounds, etc...
Member, Institute for Cancer Research,
Fox Chase Cancer Center, Philadelphia, PA
More information about the Immuno