need protocol to isolate nuclei from lymphocytes

[michelle tourigny]

I have been trying to isolate nuclei from lymph or spleen for a DNase 1
hypersensivity assay and with either 0.5% or 1% NP-40 incubation at 4
degrees C I have been unable to have consistent lysing or nuclei.  Any
suggestions?

Michelle Tourigny, grad student

-- 
Michelle Tourigny
mrtour at stud.med.cornell.edu

Elmo says:  "But I'm not a scary monster"