need protocol to isolate nuclei from lymphocytes

michelle tourigny mrtour at
Thu Oct 13 12:04:46 EST 1994

I have been trying to isolate nuclei from lymph or spleen for a DNase 1
hypersensivity assay and with either 0.5% or 1% NP-40 incubation at 4
degrees C I have been unable to have consistent lysing or nuclei.  Any

Michelle Tourigny, grad student

Michelle Tourigny
mrtour at

Elmo says:  "But I'm not a scary monster"

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