need protocol to isolate nuclei from lymphocytes

michelle tourigny mrtour at stud.med.cornell.edu
Thu Oct 13 12:04:46 EST 1994


I have been trying to isolate nuclei from lymph or spleen for a DNase 1
hypersensivity assay and with either 0.5% or 1% NP-40 incubation at 4
degrees C I have been unable to have consistent lysing or nuclei.  Any
suggestions?

Michelle Tourigny, grad student

-- 
Michelle Tourigny
mrtour at stud.med.cornell.edu

Elmo says:  "But I'm not a scary monster"



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