agglutinated monoclonals

I. MŸller muller.4 at nd.edu
Tue Oct 18 12:22:48 EST 1994


Hi, netters
we have a problem with purified (protein G) monoclonal antibodies. After
thawing, they start to agglutinate. Is there any possibility to get them
back in solution without destroying the acitivity? We did try:
a) - sonication, no success,
b) - increase salt concentration - no success
c) - addition of 0.01% detergent - no success

any suggestion is welcome.  



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