Pharmingen IL-4 Sandwich ELISA - High Background Probs

yarosh at yarosh at
Mon Oct 17 18:08:43 EST 1994

We've been working on the sandwich ELISA for both IL-4 and IFN gamma using Pharmingen reagents on Immulon 2 plates. The IFN gamma works no problem but we've
had to play around with conditions. The IL-4 ELISA gave us high backgrounds
no matter what we tried and we're getting ready to give up. Although the
Pharmingen IL-4 MAbs work great in an ELISPOT... if that's any consolation.

Oksana Yarosh

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