PBMC cell surface biotinylation protocol ?? (fwd)

Hao Xiao haoxiao at cc.UManitoba.CA
Tue Oct 25 19:23:13 EST 1994


Zafer Yildirim writes:
> From BIOSCI-REQUEST at net.bio.net Mon Oct 24 19:45:29 1994
> To: immunology at net.bio.net
> From: zxy5 at po.CWRU.Edu (Zafer Yildirim)
> Subject: PBMC cell surface biotinylation protocol ??
> Date: 25 Oct 1994 00:21:16 GMT
> Message-ID: <38hj1s$mu3 at usenet.INS.CWRU.Edu>
> NNTP-Posting-Host: roo.ins.cwru.edu
> 
> 
> Does anybody have a protocol for biotinylation of peripheral 
> blood mononuclear cells ?
> 
> Thanks in advance.
> 
> 
> 

for specific target? if not, thiol (HS-) targeting (N-maleimide derivatized
biotin, from sigma) biotinylation is considered to be mild,
efficient reaction for cells. in brief, disolve the activated biotin in
DMSO then add to you cell suspensiion at pH 7 (NB:DMSO<5% in total
volume) at room temperature for 6-12 hr. 



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