HSA Eliza

Sorin Damian sorind at crash.cts.com
Wed Oct 26 00:47:04 EST 1994


Problem: too much nonspecific binding during Eliza of Human Serum Albumin 
(HSA) in hepatocyte culture.
I am using the HSA MoAfrom sigma , the secondary is a peroxidase 
conjugated IgG. I have far FAR to much nonspecific binding of the primary 
anti HSA MoA to the plastic (or maybe the conc ratio between 
antigen/primary , or btw primary and secondary is not the right one...
Any help ?
I can give more details of the experimental approach for those 
able/willing to post their experience.
Any one out there working on HSA quantitation by ELIZA?




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