HSA Eliza

David Peritt Peritt_d at a1.mscf.upenn.edu
Wed Oct 26 10:40:24 EST 1994


Subject: HSA Eliza 
From: Sorin Damian, sorind at crash.cts.com
Date: Wed, 26 Oct 1994 05:47:04 GMT
In article <Cy9MqH.BuF at crash.cts.com> Sorin Damian, sorind at crash.cts.com
writes:
>Problem: too much nonspecific binding during Eliza of Human Serum
Albumin 
>(HSA) in hepatocyte culture.
>I am using the HSA MoAfrom sigma , the secondary is a peroxidase 
>conjugated IgG. I have far FAR to much nonspecific binding of the
primary 
>anti HSA MoA to the plastic (or maybe the conc ratio between 
>antigen/primary , or btw primary and secondary is not the right one...
>Any help ?
>I can give more details of the experimental approach for those 
>able/willing to post their experience.
>Any one out there working on HSA quantitation by ELIZA?


I can not help you at all.  I just wanted to comment that the word ELISA
is an achronym for enzyme linked immuno-sorbant assay.  Therefore the Z
is incorrect, even though it is pronounced that way. Good luck with your
query.



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