Peritt_d at a1.mscf.upenn.edu
Wed Oct 26 10:40:24 EST 1994
Subject: HSA Eliza
From: Sorin Damian, sorind at crash.cts.com
Date: Wed, 26 Oct 1994 05:47:04 GMT
In article <Cy9MqH.BuF at crash.cts.com> Sorin Damian, sorind at crash.cts.com
>Problem: too much nonspecific binding during Eliza of Human Serum
>(HSA) in hepatocyte culture.
>I am using the HSA MoAfrom sigma , the secondary is a peroxidase
>conjugated IgG. I have far FAR to much nonspecific binding of the
>anti HSA MoA to the plastic (or maybe the conc ratio between
>antigen/primary , or btw primary and secondary is not the right one...
>Any help ?
>I can give more details of the experimental approach for those
>able/willing to post their experience.
>Any one out there working on HSA quantitation by ELIZA?
I can not help you at all. I just wanted to comment that the word ELISA
is an achronym for enzyme linked immuno-sorbant assay. Therefore the Z
is incorrect, even though it is pronounced that way. Good luck with your
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