ELISA assistance

[Hurley, David Biol]

Hello Bionetters:
      We are trying to develop an ELISA for measuring porcine 
(Miss Piggy) immunoglobulin.  Our design is: Adhere the detection 
monoclonal antibody, an anti-Fc, to the plate.  Treat with the Ab 
containing serum and detect with a biotin labeled polyclonal anti-Fab.  
     Any suggestions for increasing the specificity is greatly welcome.  
Please send them to:  Hurleyd at mg.sdstate.edu