Your favorite assay for IL-6?

Michiaki Masuda masuda at
Sat Oct 29 23:12:03 EST 1994

    I am attempting to measure the amount of IL-6 in the culture supernatant
of certain rat cells. There seem to be several assay methods for the
quantitative analysis of IL-6 including a bioassay using IL-6-dependent
cells and commercial ELISA kits. 

    I wonder if there is any good fool-proof method that could give me 
a result with a publishable reliability. I'd appreciate it if you folks
could let me know about your experience and give some advice.

    Thank you.

Michiaki Masuda
Visiting Associate
Laboratory of Molecular Oncology
National Cancer Institute
Frederick, MD 21702-1201
E-mail: Masuda at

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