antibody biotinylation

Frederick Garbrecht FRED at bmt.mcw.edu
Mon Oct 31 11:58:18 EST 1994


> To:            immunology at net.bio.net
> From:          mkuiper at hgmp.mrc.ac.uk (Mr. M. Kuiper)
> Subject:       antibody biotinylation
> Date:          31 Oct 1994 16:14:55 -0000

> Dear immuno-netters,
> 
> Can anybody out there tell me whether it is possible to
> biotinylate antibodies yourself. If anyone could suggest
> an efficient protocol I would be most grateful. I want
> to use the biotinylated antibodies in ELISAs.
> 
> Thanks for your help,
> *******************************************************************
> *                                                                 *
> *	Marcel Kuiper                                             *
> *	King's College School of Medicine & Dentistry             *
> *	Department of Molecular Medicine                          *
> *	Rayne Institute, 2nd floor                                *
> *	123 Coldharbour Lane                                      *
> *	London SE5 9NU, UK                                        *
> *	Fax:	44-71-733 3877                                    *
> *	Email:	mkuiper at hgmp.mrc.ac.uk                            *
> *		rchz103 at bay.cc.kcl.ac.uk                          *
> *                                                                 *
> * 'The first myth of management is that it exists'. Murphy's Law. *
> *                                                                 *
> *******************************************************************
> 
> 
Yes, its pretty easy to do.  There is a protocol in Lane, 
"Antibodies: A Laboratory Manual".  If you don't have access to the 
book, let me know and I'll emai you the protocol I use.

Frederick C. Garbrecht
fred at bmt.mcw.edu
Bone Marrow Transplant Program
Medical College of Wisconsin
phone 414 257 5053
fax   414 257 7994



More information about the Immuno mailing list