comments of exp. plan

opt018 opt018 at nof.abdn.ac.uk
Fri Sep 2 07:32:58 EST 1994


Hi netter,

	I am planning a small experiment Re: immuno-suppression against
experimental autoimmune uveoretinitis (EAU)


The experiment is based on the following facts: 
1. 	I have induced the immuno suppression against rat EAU by giving them
	retinal antigen intranasally BEFORE the immunisation with same retinal 
	antigen.

2.	The protocol of this intranasal suppression/tolerance is to give rats
	retinal antigen intranasally daily for 2 weeks, leave the rats for a
	week without any treatment to let the antigen being processed. If you 
	then challenge the rats, you get a suppressed the EAU.

3.	I have found out that if the retinal antigens are given AFTER the 
	immunisation, it failed to protect the animals. 

4.	Cyclosporin A can induce a delayed onset of EAU, if CsA is given from
	day7 to day 15 after immunisation. The effect of CsA lasts for about a
	week.


What I want to know is, "How can I apply this self antigen
immuno-suppression/tolerance in a manner similar to clinical cases?" In another
word, does it have any significance to those subjects who are already sensitised
beforehand? 

Here is the small experiment I want to do. But some how I get puzzled about the
timing of CsA and retinal antigen administration. 

Any comments or advice? 

It is really just a matter of a race between CsA and retinal antigen. The
retinal antigen challenge is that "GO" signal!

Has anyone done the similar work and would like to share
experience and opinions?  

++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
Legend
	Challenge with retinal antigen		!
	RE intranasal tolerisation 		**
	CsA administration			^^


	Day 0		Day 7		Day 14		Day 21		...
----------|---------------|----------------|---------------|------------------
GROUP

A 	 ! *********************************
 	  		   ^^^^^^^^^^^^^^^^^

B 	 ! *************************************************
	  		   ^^^^^^^^^^^^^^^^^

C 	 ! *********************************
			   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^

D 	 ! **************************************************
			   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^

E 	 ! 		   ^^^^^^^^^^^^^^^^^


F 	 !		   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^


G 	 !


+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++

Cheers!

Y-F Cheng,MB ChB
Dept. of Ophthalmology
Univ. Aberdeen

<opt018 at aberdeen.ac.uk>



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