separat. hi-low affin Ab

Rubbi C P rubbc at solb1.essex.ac.uk
Wed Sep 7 06:49:40 EST 1994


In article <9409040549.AA21948 at mira.cc.umanitoba.ca> haoxiao at CC.UManitoba.CA writes:
>Approach two:
>Gradient chaotropic detachment of bound Ab (pH 4.5 to 2, salt conc. low to
>high, %organic sohlvent low to high). Low affin Ab should be more readily
>detached than the high affin one. 
That's been done with ammonium thiocyanate and called something like
"ranking of affinities". I'll try to find the reference(s).
BTW: it works!

Carlos



More information about the Immuno mailing list