Biotinylation of Ab

BiotecBurd biotecburd at aol.com
Fri Sep 9 18:23:01 EST 1994


In article <prudence.grandison-090994160516 at 202.36.157.201>,
prudence.grandison at genesis.co.nz (Prudence Grandison) writes:

Usually an equal molar ratio of biotin to protein works well when using
activated biotin molecules (e.g., biotin- carboxy- methyl-N-
hydroxyusuccinimide ester).  If you find that you are getting high
background using biotin/streptavidin systems, dry labelling your protein
with dig-NHS.  The anti-dig Fab conjugates do not stick non-specifically
and result in low background.

Good luck.

Dave Burden
Biotechnology Training Institute



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