Biotinylation of Ab

GDWX gdwx at aol.com
Fri Sep 9 19:54:01 EST 1994


In article <34qqol$4ck at search01.news.aol.com>, biotecburd at aol.com
(BiotecBurd) writes:

Subject: Re: Biotinylation of Ab
From: biotecburd at aol.com (BiotecBurd)
Date: 9 Sep 1994 19:23:01 -0400
Message-ID: <34qqol$4ck at search01.news.aol.com>

In article <prudence.grandison-090994160516 at 202.36.157.201>,
prudence.grandison at genesis.co.nz (Prudence Grandison) writes:

>>Usually an equal molar ratio of biotin to protein works well when using
activated biotin molecules (e.g., biotin- carboxy- methyl-N-
hydroxyusuccinimide ester).  If you find that you are getting high
background using biotin/streptavidin systems, dry labelling your protein
with dig-NHS.  The anti-dig Fab conjugates do not stick non-specifically
and result in low background.<<

What is the [dig] in [dig-NHS]?  I am always looking for ways to reduce
background.  Much obliged.

Geoffrey D. Wheelock
Ithaca, NY



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