Obtaining anti-human IgE for elisa spot assay

N Rhodes mb1nr at sunc.sheffield.ac.uk
Mon Sep 12 11:17:45 EST 1994

Christopher Spry (cspry at place.demon.co.uk) wrote:
: A colleague is setting up an assay to count human lymphocytes secreting
: IgE to a defined antigen using an Elisa spot assay. He would like to
: know if there are any anti-IgE antibodies that he could purchase or
: obtain to do this. The indicator system he plans to use is an alkaline
: phosphatase-linked second antibody. Please email him (Dr. David Lewis)
: if you can help: sgjf300 at sghms.ac.uk

: Thanks for any help you can give.

: Christopher Spry

Try Dakopatts.   Peroxidase conjugated rabbit Igs to human IgE, epsilon
chains.   Cat number P295.   Always worked very well for us!   They 
probably also sell a AP linked version.

good luck!


Nick Rhodes
Krebs Institute for Biomolecular Research,
Department of Molecular Biology and Biotechnology,
PO Box 594,   Firth Court,   University of Sheffield,
Western Bank,   Sheffield,   South Yorkshire,
S10 2UH,   United Kingdom.

N.Rhodes at sheffield.ac.uk
mb1nr at sunc.shef.ac.uk

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