High and low ionic force

GDWX gdwx at aol.com
Wed Sep 14 17:24:13 EST 1994


In article <9409140009.AA12443 at infomed>, monoclon%infomed at GN.APC.ORG (Ctro
Inmunologia Molecular) writes:

Check out *Antibodies, a laboratory manual,* by Harlow and Lane. (I am
putting this down from memory so if I got the cite wrong, forgive me)  If
you know the subclass of your monoclonal, H&L will tell you if it will
stick to protein A.  There is also a method in H&L for hi/low salt
isolation of polyclonals on protein A that may be applicable.  You might
want to consider protein G also.  Hope this helps
Geoffrey D. Wheelock
Ithaca NY   



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