Looking for a very tight binding ab/epitope pair

Jerry Woodward woodward at seqanal.mi.uky.edu
Wed Sep 21 13:10:38 EST 1994


WEDEL at vms.biochem.mpg.de wrote:
: This is my virgin posting; please excuse any errors in nettiquete.
: I am looking for an antibody/epitope pair that bind very tightly with which 
: I can stably link the antibody to an in vitro synthesized protein.  For my 
: purposes the half life of the interaction under standard conditions would 
: have to be more than an hour.
: Does anyone know:
: 1) of such an antibody/epitope pair?
: 2) someone who might know?
: 3) a good place to look, or a better news group for me to post to with this 
: question?

: Thanks,
: Andrew Wedel      wedel at vms.biochem.mpg.de


Why not use avidin-biotin?  Kits are widely available for conjugating and the 
affinity is much higher than any Ag-Ab.

Jerold G. Woodward, Ph.D.
Associate Professor 
Department of Microbiology and Immunology
University of Kentucky Medical Center
Lexington, KY 40536-0084
Phone: (606) 323-5538
FAX: (606) 257-8994
e-mail: woodward at seqanal.mi.uky.edu
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