Staining SDS-PAGE gels without fixing proteins

Richard J. Shaw shawr at
Tue Sep 27 12:00:01 EST 1994

A suitable method can be found in the excellant lab manual "Antibodies" by E. 
Harlow and D. Lane p61-62.  They suggest only fixing & staining a strip of the 
gel, then lining up this with untouched part of gel.  Also if you want to 
modify your antigen as little as possible, try running PAGE gels with other 
detergents than SDS.

Richard Shaw
Technician AgResearch Wallaceville

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