IEF problems/ MAbs

Ctro Inmunologia Molecular monoclon%infomed at GN.APC.ORG
Fri Sep 30 23:35:05 EST 1994

   Does anybody know how can detect by IEF problems with monoclonal 
   antibody like deamidation,loses of carbohydrates,glycosilation and 
   others.If my protein's isoelectric point (pI)  changes, how can I 
   say which one of these mecanism are responsible for pI change?
   Advices,references and practical sugestions will be welcome.
   Thanks in advance
   monoclon at   

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