Protein electrophoresis pattern
Ctro Inmunologia Molecular
monoclon%infomed at GN.APC.ORG
Fri Sep 30 23:30:54 EST 1994
I am obtaining different electrophoresis pattern for a monoclonal
antibody in SDS- PAGE when I run gels in conventional system and
stain with coomasie ( Phast Gel Blue from pharmacia ) and when I run
PhastSystem gel stainig with Silver.the problem is that I obtain
several bands in conventional system and just one band with
PhastSystem, also I obtain a normal pattern for this monoclonal
antibody by IEF and NATIVE- PAGE with PhastSystem.
Where those extrabands come from?
If this extrabands are not artifacts Why I can not detect this band
by silver stain in phastSystem.
Advices and sugestions will be welcome.
Thanks in advance.
monoclon at infomed.sld.cu
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