Black ECL blots

James Stiehr jcim at
Tue Apr 4 12:04:58 EST 1995

Has anyone else ever had problems using polyclonal antisera on a western
blot detected with ECL resulting in an overall black blot.  It is almost as
if the blots were not blocked at all (they were).  The ECL supplier didn't
have any suggestions.  If anyone has come across this problem and hopefully
a solution, I would appreciate any suggestions. 
James Stiehr

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