Il-12 bioassay in mice

davis at davis at
Tue Apr 4 23:27:05 EST 1995

In article <3ld31l$tuq at>, aredwood at (Alec 
Redwood) writes:
> Hi just a quick question is there an established protcol for the 
> measurement of murine IL-12, there are methods for human IL-12, but I 
> need to assay murine IL-12.  Any advise please.

Murine IL-12 is active on human cells.  What are you measuring?  If your 
sample does not contain other cytokines that act on human cells, you could
use a day 4 PHA-blast assay as described previously (Tahara et al.  Cancer
Research 54: 182-189, 1994).  If you are looking at mouse serum you might
be in trouble.

Ian Davis					davis at
Ludwig Institute for Cancer Research
Melbourne Tumour Biology Branch.

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