Il-12 bioassay in mice

Jordan Orange Jordan_Orange at Brown.edu
Wed Apr 5 16:09:30 EST 1995


Alec,
  IL-12 biological assays in mice can be performed.

The most sensitive measure of IL-12 in any biological sample
is IFN-gamma induction from responder cells.

This is orders of magnitude more sensitive than IL-12-induced
proliferation.

The IFN-gamma induction assay for murine samples has been published.
see Heinzel, et. al. (1994) Infect-Immun. 62(10):4244.

I laud your efforts to find a biological assay for IL-12 as the
heterodimeric nature of this cytokine necessitates demonstration
of biological activity in order to determine induction of factor.

Jordan S. Orange
Division of Biology and Medicine
Brown University
Providence RI, 02912



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