Black ECL blots

U12201 at uicvm.uic.edu U12201 at uicvm.uic.edu
Thu Apr 6 16:38:03 EST 1995


Use antibodies at around 1:10,000 or more.
Use good blockers (SuperBlock from Pierce)
Dilute, dilute, dilute  ! ! !
In article <3lru7q$pku at pipe3.nyc.pipeline.com>, jcim at nyc.pipeline.com (James Stiehr) says:
>
>Has anyone else ever had problems using polyclonal antisera on a western
>blot detected with ECL resulting in an overall black blot.  It is almost as
>if the blots were not blocked at all (they were).  The ECL supplier didn't
>have any suggestions.  If anyone has come across this problem and hopefully
>a solution, I would appreciate any suggestions.
>
>Regards
>James Stiehr



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