Need method for isolating pure T from Human PBLs

David Peritt Peritt_d at a1.mscf.upenn.edu
Fri Apr 7 13:23:15 EST 1995


In article <3m2io4$b3a at decaxp.harvard.edu> Derek V. Chan,
chan4 at fas.harvard.edu writes:
>Anybody know of a good, easy way to purify T-cells from PBLs?  We want 
>to remove NK cells and any other cells that may lead to nonspecific
lysis of
>target cells in a CTL assay.  We've utilitzed Percol T gradient
separation;
>however, it appears that the population we have isolated is still not
totally
>pure T....


I assume you have pondered the use of the classical techniques such as
negative or poistive panning, complement killing and FACS/MACS sorting.



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