Need method for isolating pure T from Human PBLs

[Derek V. Chan]

Anybody know of a good, easy way to purify T-cells from PBLs?  We want 
to remove NK cells and any other cells that may lead to nonspecific lysis of
target cells in a CTL assay.  We've utilitzed Percol T gradient separation;
however, it appears that the population we have isolated is still not totally
pure T....

Derek V. Chan
chan4 at fas.harvard.edu
dvchan at netcom.com