Black ECL blots

Dennis Vik dvik at
Mon Apr 10 18:07:57 EST 1995

> Has anyone else ever had problems using polyclonal antisera on a western
> blot detected with ECL resulting in an overall black blot.  It is almost as
> if the blots were not blocked at all (they were).  The ECL supplier didn't
> have any suggestions.  If anyone has come across this problem and hopefully
> a solution, I would appreciate any suggestions. 

Are you using diluted serum? If so, it might help to purifiy the antibody
and use that directly. Hope this helps.


Dennis_Vik at

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