Need method for isolating pure T from Human PBLs

[Calvin and Hobbbes]

In article <3mj9sd$igc$1 at mhadg.production.compuserve.com>, G R Barclay
<100066.300 at CompuServe.COM> wrote:

> Ever tried sheep-red cell rosetting, then layering over 
> lymphocyte separation medium and collecting what goes to the 
> bottom of the centrifuge tube? 
> 
> -- 
> GRB

Actually, You would pull down all of the T-cells through the adhesivity of
Niaminadase treated SRBC, Then how would you remove the SRBC from the all
important T-cells? Even if you could remove the SRBC, I would think that
the surface markers for the T-cells would be altered or damaged and would
affect the cells adversely. The only true method which would work with
lower efficiency would be Dynal CD marker beads(positve selection) or
glass-wool columns. Both of these methods would be best when considering
selectivity and cell viability.

Good luck!

Jon Stacks