Black ECL blots

AShaw5 ashaw5 at
Sun Apr 23 15:40:02 EST 1995

I would 
1.  Check you blocking conditions and your second antibody.  I would try
different blocking proteins namely, increase BSA and add milk.
2.  Try decreasing the concentration of the secondary antibody.  Because
of the increased sensitivity of ECL, I find that we generally have to
dilute the antibody 1/1000 or more to get a nice blot.  
good luck.
Andrey Shaw

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