Deglycosylating IgG

U12201 at uicvm.uic.edu U12201 at uicvm.uic.edu
Tue Apr 25 08:31:50 EST 1995


What I have done is to simply make Fab or Fab2 fragments
using the Pierce kits - since the carbs are on the Fc part
- pronto you have deglycosylated IgG.

In article <3n6ddn$loi at phase.helix.net>, sansell at helix.net (Steven Ansell) says:
>Does anyone have any experience or references for protocols for
>deglycosylating
>antibodies, specifically IgG?
>
>Any (useful!) suggestions would be appreciated.
>
>Thanks,
>
>Steve Ansell.



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