Deglycosylating (?????) IgG

Iain Wilson wilson at edv1.boku.ac.at
Tue Apr 25 09:37:06 EST 1995


ANDREI POPOV <ANDREI.POPOV at bbsrc.ac.uk> wrote:
>
> >Does anyone have any experience or references for protocols for deglycosylating
> >antibodies, specifically IgG?
> 
> >Any (useful!) suggestions would be appreciated.
> 
>                           useful???????
>                          
> Try tunicamycin
>                        

Tunicamycin will not deglycosylate - it merely prevents formation of
dolichol-linked oligosaccharides - and so is a biosynthetic inhibitor.
The partially-cryptic site of IgG oligosaccharides would create 
difficulties in using enzymatic deglycosylation, whilst using non-
denatured IgG.

Iain
wilson at edv1.boku.ac.at



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