Deglycosylating (?????) IgG
ANDREI.POPOV at bbsrc.ac.uk
Tue Apr 25 16:06:56 EST 1995
> >Does anyone have any experience or references for protocols for
> >antibodies, specifically IgG?
> >Any (useful!) suggestions would be appreciated.
> Try tunicamycin
<Tunicamycin will not deglycosylate - it merely prevents formation of
<dolichol-linked oligosaccharides - and so is a biosynthetic inhibitor.
Thanks for the info
<The partially-cryptic site of IgG oligosaccharides would create
<difficulties in using enzymatic deglycosylation, whilst using non-
Sorry, but I could not understand this...
So far I have not heard any complaints about the usage
of the drug
More information about the Immuno