Plasma to serum protocols?

Robertson Davenport rddvnprt at umich.edu
Tue Aug 1 08:00:13 EST 1995


The method for clotting plasma will depend on the anticoagulant. The 
following will work for donor plasma anticoagulated with ACD, CPD or 
CPD-A1.

Reagents:
Bovine thrombin 500 units/ml
Calcium chloride 10%wt/vol

Method:
Add 0.25 ml CaCl2 per 100 ml plasma.
Add 0.2-0.4 ml thrombin per ml plasma
Mix well and allow to clot at room temp. for at least 4 hours.
Freeze converted plasma at -20C overnight.
Thaw at room temp.
Centrifuge at 5000g for at least 5 min.
Remove serum and store at -20C until required.

Reference:
W. John Judd. Methods in Immunohematology. 2nd. Ed., Mongomery Scientific 
Publications. Durham, NC.

-- 
Robertson D. Davenport, MD
Assistant Professor                    Associate Medical Director
Department of Pathology                Blood Bank and Transfusion Service
University of Michigan Medical School  University of Michigan Hospitals
rddvnprt at umich.edu





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