Plasma to serum protocols?

Robertson Davenport rddvnprt at
Tue Aug 1 08:00:13 EST 1995

The method for clotting plasma will depend on the anticoagulant. The 
following will work for donor plasma anticoagulated with ACD, CPD or 

Bovine thrombin 500 units/ml
Calcium chloride 10%wt/vol

Add 0.25 ml CaCl2 per 100 ml plasma.
Add 0.2-0.4 ml thrombin per ml plasma
Mix well and allow to clot at room temp. for at least 4 hours.
Freeze converted plasma at -20C overnight.
Thaw at room temp.
Centrifuge at 5000g for at least 5 min.
Remove serum and store at -20C until required.

W. John Judd. Methods in Immunohematology. 2nd. Ed., Mongomery Scientific 
Publications. Durham, NC.

Robertson D. Davenport, MD
Assistant Professor                    Associate Medical Director
Department of Pathology                Blood Bank and Transfusion Service
University of Michigan Medical School  University of Michigan Hospitals
rddvnprt at

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