Looking for peptide-immunization protcol to elicit CD4 CTLs

zcn zcn at ahabs.wisc.edu
Tue Aug 8 16:53:12 EST 1995


I'm currently involved on a project attempting to elicit CD4 CTLs in
BALB/c specific to epitope-possessing synthetic peptides.  To date we have
immunized mice by i.p. injection of peptide/CFA followed by 2 additional
injections of peptide/IFA.  Injections were approx. 1 wk. apart. 
Secondly, we tried immunizing by i.v. injections of peptide/PBS.  Again,
we used a total of 3 injections approx. 1 wk. apart.  Both protocols used
peptide at 100ug/injection.  These attempts appear unsuccessful.

Would appreciate any information to head us in the right direction.

Zane Neal
Univ. Wisc.-Madison
Dept. of Animal Health and Biomedical Sciences 
zcn at ahabs.wisc.edu



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