polyclonal antibodies in mice

David Peritt Peritt_d at a1.mscf.upenn.edu
Tue Aug 22 07:44:56 EST 1995

In article <Pine.OSF.3.90.950822145105.20760A at central.murdoch.edu.au>
Vidyani Manatunja, vidyani at CENTRAL.MURDOCH.EDU.AU writes:
>How much antibody can I expect to collect from the blood serum  
>of one mouse?

If you are good you will get maximum 0.7 ml of sera but you will have to
squeeze the mouse dry.  I suggest several mice.  Injection protocols are
as numerous as the sands on a beach.  The gold book has a good one
(current protocols in Immunology).

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