high-titer rabbit Abs
Joseph A. Price, Ph.D.
price at vms.ocom.okstate.edu
Wed Aug 23 11:34:38 EST 1995
In article <1995Aug22.142250.5955 at inet.d48.lilly.com>,
qx01940 at inet.d48.lilly.com says...
>Despite several successes in the past 10 years I am encuntering problems
>raising high-titer and high-affinity abs in rabbits using fusion
>E. coli. We have immunized 100ug of protein in CFA followed by 100ug in
>100ug in PBS in three week intervals. Injections have been subscapula.
>scheme generally gives us high titer sera. Knowing that rabbit
>generation is somewhat of a black box, I am stuck.
>I would appreciate some suggestions on boosting to up the titer.
>Thanks in advance,
CFA is often adaquate, but several adjuvants, generally much better, are
available on the market. Species effects vary as does sucess with
specific antigen, you may need to evaluate them in rabbits for your
protein. RIBI has one, I have seen others but I forget the sources.
Joseph A. Price, Ph.D
PRICE at VMS.OCOM.OKSTATE.EDU
Ph 918 561-8231
FAX 918 561 8412
Dept. Biochem. & Micro.
COM-OSU 1111 W.17th. St.
Tulsa OK 74107
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