transfection of PBMs

john sinclair js at mole.bio.cam.ac.uk
Fri Aug 25 03:12:53 EST 1995


Does anyone have a reproducible method to transiently transfect 
purified peripheral blood monocytes ?
We have tried lipofectin, DEAE-Dextran, Calcium phosphate
and electroporation but none of these give high enough levels
of reporter gene expression using a very strong promoter.
Any suggestions will be gratefully received.
Thanks.
John S.



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