Looking for peptide-immunization protcol to elicit CD4 CTLs
drummond at ncifcrf.gov
Tue Aug 22 11:07:28 EST 1995
In article <zcn-0808951553120001 at pokey.ahabs.wisc.edu> zcn at ahabs.wisc.edu (zcn) writes:
>I'm currently involved on a project attempting to elicit CD4 CTLs in
>BALB/c specific to epitope-possessing synthetic peptides. To date we have
>immunized mice by i.p. injection of peptide/CFA followed by 2 additional
>injections of peptide/IFA. Injections were approx. 1 wk. apart.
>Secondly, we tried immunizing by i.v. injections of peptide/PBS. Again,
>we used a total of 3 injections approx. 1 wk. apart. Both protocols used
>peptide at 100ug/injection. These attempts appear unsuccessful.
>Would appreciate any information to head us in the right direction.
>Dept. of Animal Health and Biomedical Sciences
>zcn at ahabs.wisc.edu
Try giving the peptide in sub-cutaneous injections. Give the first in Complete Freunds and subsequent ones in Incomplete Freunds. Off the top of my head I would u
use the peptide at about 100 ug/ml and inject up to 50 ul.
Drummond at avpvx1.ncifcrf.gov
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