polyclonal antibodies in mice

Bio Bone biobone at aol.com
Sat Aug 26 22:41:41 EST 1995


I have routinely generated polyclonal ascites in mice by immunizing
pristane primed mice IP with antigen/adjuvant (RIBI) then once the sera
titers are high, you can inject mouse myloma cells (SP2/0) IP and the
mouse generates typical amounts of polyclonal ascites that have titers
equivelent to those found in the seras.

Bradley Bone
COR Therapeutics, Inc.



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