Need help with T-cell proliferation assay

Tom Thatcher ttha at uhura.cc.rochester.edu
Tue Dec 12 18:50:27 EST 1995


OK, here's the scoop.  I am trying to do a T cell proliferation assay
where I immunize mice with a protein, remove the draining lymph
nodes, and culture the T cells with antigen.  Proliferation is 
measured by 3H thymidine incorporation.  If I use the protein as
the in vitro antigen, it works fine.  If I use a synthetic peptide
of the protein (one that works for other labs and for a prior grad
student in my lab) it doesn't work.  I'm really beating my head on
the wall with this and I'd appreciate any suggestions at all.

Thanks

-- 
Tom Thatcher                          | You can give a PC to a Homo habilis,
University of Rochester Cancer Center | and he'll use it, but he'll use it
ttha at uhura.cc.rochester.edu           | to crack nuts.



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