Need help with T-cell proliferation assay
parkerd at ohsu.edu
Wed Dec 13 17:51:20 EST 1995
In article <1995Dec12.235027.25140 at galileo.cc.rochester.edu>,
ttha at uhura.cc.rochester.edu (Tom Thatcher) wrote:
> ...I am trying to do a T cell proliferation assay.....
> If I use the protein as
> the in vitro antigen, it works fine. If I use a synthetic peptide
> of the protein (one that works for other labs and for a prior grad
> student in my lab) it doesn't work...
Are you using the same peptide preparation as the former student? Some
peptides are not what they are supposed to be, and the T cells can tell!
David C. Parker
Oregon Health Sciences University voice: (503)494-1498
Molecular Microbiology & Immunology fax: (503)494-6862
3181 SW Sam Jackson Pk Rd, L220 internet: parkerd at ohsu.edu
Portland, Oregon 97201-3098
More information about the Immuno