I am looking for protocol suggestions to induce tolerance in rabbits to
a complex extract from rat liver. I would like to try to do subtractive
immunization, in an effort to raise antisera that will recognize the
different antigens present in another complex extract from liver that I
know contains a subset of newly expressed proteins. I am having
difficulty locating references that would help me to design such a
scheme, and would appreciate any assistance you could offer, on such
topics as suggested antigen concentration, inoculation schedules, use of
cyclophosphamide to deplete activated T-cells, etc. Also it seems that
I would need to simultaneously maintain tolerance to the first extract,
while inducing an immune response to the second. What are the
limitations involved?
Thanks in advance for your help.
Please respond here or by E-mail
Jennifer L. Cruise, Dept. of Biology, University of St. Thomas
JLCRUISE at STTHOMAS.EDU
