Detect low expression cell surface antigens

Garry P. Nolan gnolan at cmgm.stanford.edu
Thu Dec 14 22:32:15 EST 1995


Hello

First thing to do, assuming you are detecting by FACS, is to learn 
how to do autofluorescence compensation.  Essentially this is an 
electronic process that allows subtraction of background.  Very 
effective.  Paper on how to do it is Albert and Herzenberg.  Then, 
use a secondary antibody that is APC or PE conjugated.  These are 
currently the brightest dyes for detection by FACS.

Good luick.

Garry P. Nolan



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