I am about to make antigen specific T cell hybridomas after imunization with a
peptide.
What is the frequency of Ag specific hybridomas I can expect if I start with
the draining lymph node and a 3-day restimulation in vitro with the peptide ?
Is there a way (facs sorting ?) to select for T cells that have responded in
vitro before doing the fusion? Would that help anyway ?
Thanks for your advices
