ADCC protocol

SLRCmg slrcmg at aol.com
Fri Dec 15 20:23:45 EST 1995

Alex wrote:

>Has anyone got a good ADCC prtocol using nucleated mouse cells as the
>Any help would be appreciated.

If you're using human efffctor cells, one easy way would be to use
hybridoma cells opsonized with rabbit anti-mouse-Ig.  Rabbit Fc gamma is
compatible with the human Fc gamma receptor III (CD16) which mediates
ADCC.  Just don't use hybridomas producing antibodies to human antigens,
and make sure you wash out any secreted antibody before performing the

Hope this helps


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