Hi! I would like to refer you to our paper, you will find a brief description concerning how to generate antigen specific T cell hybrids. Please don't hesitate to contact me if you still have more questions. Osman et. al. 1993. J. Exp. Med. 177:387-395 Good Luck! Gamal E. Osman, Ph. D. University of Washington gamal at u.washington.edu